发信人: moses (Moses), 信区: Biology
标 题: Re: 提取genomic DNA for PCＲ求助
发信站: BBS 未名空间站 (Sun Nov 19 23:57:57 2006)

for tail DNA genotyping: Here is my protocol

1. 600ul 50mM NaOH
2. 100oC 10min
3. 50ul pH8.0 1M Tris-HCl
4. 0.2-1ul for PCR in 15-25ul volume

Our lab has been using this for many years.



【 在 WNT (傻福) 的大作中提到: 】
: A simple protocol:
: add 100ul 50mM NaOH in a 1.5 tube
: clip toes or tails(a little one)and put in NaOH
: 95 C for 1 hour
: add 10ul 1M tris(ph=5.0)
: 1-1.5ul for PCR
: It works great for me and I have done thounds of PCR.
: prep



--

※ 来源:·BBS 未名空间站 http://mitbbs.com·[FROM: 68.250.]