发信人: coconut (可可), 信区: Biology
标 题: Re: why this E.Coli grow so slow?
发信站: The unknown SPACE (Thu Jul 13 19:08:03 2000), 站内信件


Perfectly normal.

The bacterial was not in growth phase. It could be caused by
1. high cell density. O.D. can mislead you sometimes because
that can vary for different strains. But don't people
usually use OD 600 (598) to check cell density?
2. the cells were in cold for a period of time.
3. lack of oxygen.

【 在 whoknowit (冰毒) 的大作中提到: 】
: I transformed a two antibiotic resistant( amp and rifamp) plasmid into E.coli
: BL21(DE3)PlysS cells, and grow it. I found it grows very slow.
: it takes more than one hour for the OD Changing from 0.6 to 0.7 at 546nm.
: I also checked that the slow growth of the cell has nothing to
: do with the concentration of antibiotics. it grows slow without any
: antibiotics in culture.
: it is that normal?
: I know that the antibiotic resistant cell may grow slower. But how come
: it grow so slow even without any antibiotics in culture medium.
: ///confused.


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百事皆可可，一心独悠悠

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