发信人: netbuggie (中原一点虫), 信区: Biology
标 题: Re: Who knows about RNase H? --- ask for help!
发信站: The unknown SPACE (Sun Jul 23 00:48:41 2000), 站内信件

i have only used RNaseH, RNaseT1 for cDNA stuff. and i donno
its function in mammalian.

let us see this one

Mol Cell Biol 1999 Dec;19(12):8361-71
in S.C., RNase H was found to remove RNA primer from Okazaki frag.

Biochemistry 1999 Nov 16;38(46):15097-103
for HIV and other retrovirus, RNase H function is essential for
activity of the RT.

J Biol Chem 1999 Oct 1;274(40):28270-8
Human RNase H was cloned and found to cleave DNA:RNA substrate,
then what function:)? antibody template switch? DNA replication?

Mol Pharmacol 1999 Oct;56(4):841-8
Human RNase H was inhibited by thiopurine, RNase H functions in
DNA replication.

Methods 1999 Jul;18(3):252-65
RNase L was activated by 2'-5
RNase L was activated by 2'-5' phosphodiester formation, functions
to protect mammalian, in supplement to RNase H for higher euka.

J Virol 1999 Aug;73(8):6573-81
RNase H is necessary for retrovirus.


Hehe, so the basic idea is that RNase H has been applied by virus
for infection/replication. and in higher eukaryotes, it has other
functions in DNA replication, anti-viral, and antibody formation.


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