发信人: coconut (可可), 信区: Biology
标 题: Re: 哪位大仙知道生化中K cat 的定义是什么
发信站: The unknown SPACE (Mon Nov 6 16:52:06 2000), 站内信件


kcat = k2
k1 k2
E + S -----> ES -----> E + P
<-----
k(-1)

k2 determintes how fast the ES complex is returned to the
enzyme pool. One can imagine that if an enzyme has a difficulty
to dissociate from ES, soon [E] would be depleted. k2 is usually
the slowest step and therefore determines the rate of enzymatic
reactions. Also, when talking about initial rate, assuming
that there are large amount of [S], then all enzymes are able to
bind to substrate, k2[ES] = Vmax = k2[E]initial

【 在 citytiger (小老虎~在等小兔) 的大作中提到: 】
: RT，还有它和反应速度V max， 和底物，和酶浓度有什么联系？泄了！
: 明天要交作业，刚刚开始做就打了我一棒。学校的网坏了，联不上edu
: 和gov以外的地址，还没法查破破的蛋白质基础，竟然有
: 这么多的反应动力学和热力学，弄得人人怨声载道。


--

百事皆可可，一心独悠悠

※ 来源:．The unknown SPACE bbs.mit.edu．[FROM: 169.237.218.28]