发信人: notch (甜蜜的猪笼草), 信区: Biology
标 题: Re: [Question 1] About microarray
发信站: The unknown SPACE (Tue May 29 18:48:04 2001), 站内信件

No. It's like the other way around...

Affymetrix chips use synthesized 25-base long oligonucleotides as probes,
including perfect match and mis-match probes. A set (16-20 ) of probes are
designated to detect one transcript. So all the probes on the chip stand
for known genes, which is nice for reproducibility and further analysis of
your data, while could be a limitation too: you can only detect the expression
level of the probes they provide.

I haven't worked with the so-called "arrays", but as I know, people
fragmentate the genomic DNA or cDNA (this might involve RT-PCR, not sure)
and print them to glass slides. So you can choose your own gene pool
to hybridize with, like DNA/mRNA of some specific tissue, and there could
be unknown genes too. The hard part is data analysis, which is obvious
by the way it's made. People in my lab are trying to print some libraries
and I might be able to use our own arrays in one month...As soon as they
solve the problem of data analysis. That'll be much cheaper.. hehe, and can
even work as a facility, 创收，呵呵

【 在 golem (声色犬东方必败) 的大作中提到: 】
: Don't quite know the exact tech strategy. Just talk a little
: bit by their title meaning.
: The first one seems to attach mRNA direct to Chips basing on
: some affinity fact of poly (A). If it's true, it could have
: a good represence since each mRNA is used according to their
: "original" proportion. Bad thing is the reproducibility.
: The second one may involve PCR, which will result a
: disproportional represence of mRNA library. Good thing is
: reproducibility.
: Just by guess, maybe onsense at all, hehe.
: 【 在 lxj (FreeSpace: telnet 193.11.249.37 3000) 的大作中提
: 到: 】
: : Question:
: : Compare the construction and design of Affymetrix m-RNA chips
: : with the construction and design of so called Stanford or cDNA arrays,
: : and - what are the main advantages and disadvantages with each
: : of the two systems?
: : URGENT! //bow...
--
※ 修改:．notch 于 May 29 18:50:08 修改本文．[FROM: 156.111.188.223]
: : ※ 来源:．The unknown SPACE bbs.mit.edu．[FROM: 193.11.249.37]


--
小猪小猪，一天一头
吃下肚里，我是猪笼

※ 来源:．The unknown SPACE bbs.mit.edu．[FROM: 156.111.188.223]