发信人: leohawk (leohawk), 信区: Biology
标 题: Re: Who has experience in doing In-situ Hybridization?
发信站: The unknown SPACE (Wed Jul 18 09:56:25 2001), 转信

in situ with anti-sense RNA (labeled with DIG?)or DNA FISH???

I have good experience with both, anti-sense RNA in situ can have very
high resolution, for example, if certain gene's expression is cell autonomous,
it can be shown as the only stained cell along many...

DNA FISH is harder to do, if you are observing whole mount tissue, you
probably will need a very good confocal or de-convolution microscope. I think
the quality of the probe is the most important factor...

Actually, I don't get your question, when you are doing in situ, should you
worry about the signal rather than the shape of the cell???

you should have provided more info...


【 在 superfeiyun (superfeiyun) 的大作中提到: 】
: Hi, I am doing in-situ hybridization. Unfortunately, I can
: not get the right result. I only get round shape cell, but
: all the cells should not like all same.
: Any one know which step I did wrong?
: Thanks a lot!


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