发信人: puppeteer (兵马甬), 信区: Biology
标 题: Re: Comment on the EST technology
发信站: The unknown SPACE (Wed Mar 27 13:30:04 2002) WWW-POST

Well, it depends on how long the EST is then. It also depends on the library
the EST is built from. If the normalization is not very good, you will miss
genes that are not highly expressed.
【 在 Marble (小石头哥哥) 的大作中提到: 】
: I was listening to a speech given by J. Craig Venter the other day. It was a
: very impressive one, though it was much more like elementary education to
the
: general audience. One thing that really impressed me is the expressed
sequence
: tags technology (ESTs), which was developed and applied by J. Craig Venter
to
: discover a multitude of new genes and consequently integrated into the Human
: Genome Project. The explosion of discovering new genes with the help of
: mathmatics and computers was realized only with the immerge of the EST
: technology. Basically, the current estimated genes for human beings are
about
: 26,000, and 23,000 for the Mus musculus. This number is pretty surprising to
: most biologists due to the large size of the genome, which means that almost
: 99% of the genome is "junk". The number of the known ESTs (or publicly
: available) is about 20 folds of the gene numbers. I do not know how well the
: ESTs can represent the proposed 26K genes, and hope someone could educate
more
: on this topic. My understanding is that the quality of the ESTs is
definitely
: a prerequisite for the following studies.
:
:

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