发信人: cloudwater (行云流水), 信区: Biology
标 题: Re: help,pls
发信站: The unknown SPACE (Fri May 31 00:16:42 2002) WWW-POST

I guess the apitope of the peptide will just rely on a few amino acids, so I
don't think the struture prediction is of critical importance.

If the peptied of the interest has few siquence similarity, then just use the
whole peptide to immune the rabbit or mice. however, if there is amy
siqnificant sequnce similarity to other proteins, you need to use the part
that are not conserved to immune.

BTW, if you want predict peptide structure, SWISS-PROT is a good place to
start.

Good luck.
【 在 current (可以不戒网) 的大作中提到: 】
: 想给一序列已知，功能未知的蛋白做抗体，然后荧光免疫把该蛋白在细胞内定位,抗体
怎么
: 做？
初步设想从该蛋白序列中找一段peptide,连到载体上，拿来免疫动物，这段peptide
: 怎么确定呢？有什么好的structure prediction 的软件？或者别的方法？thx
:

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