发信人: royluo (roy), 信区: Biology
标 题: Re: help,pls
发信站: The unknown SPACE (Fri May 31 19:46:37 2002), 站内信件

【 在 cloudwater (行云流水) 的大作中提到: 】
: I guess the apitope of the peptide will just rely on a few amino acids, so I
: don't think the struture prediction is of critical importance.

No, no, the problem is that the peptide you use for antigen should be
on the surface of the protein. Otherwise, your antibody is not gonna work
cause the antigen is buried inside. So knowing the general structure topology
of the protein is important. But I doubt there is a good way to predict
structure since the protein's function is unknown. If this protein has
some homologues, you can compare the different proteins and find conserved
sequences. Because conserved residues may be important surface area, using
these sequences as antigen will produce the antibody towards the surface of
the protein eventually.



: If the peptied of the interest has few siquence similarity, then just use the
: whole peptide to immune the rabbit or mice. however, if there is amy
: siqnificant sequnce similarity to other proteins, you need to use the part
: that are not conserved to immune.
: BTW, if you want predict peptide structure, SWISS-PROT is a good place to
: start.
: Good luck.


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