发信人: golem (杯杯狗愣), 信区: Biology
标 题: Re: protein identification
发信站: The unknown SPACE (Tue Jun 11 12:47:19 2002) WWW-POST

hehe, it is a basic strategy via accurate MS for proteomic level's screening
and identifying.
The precision is really dependant on how you construct the peptide map
database. Generally, they take cDNA translated amino acid sequence as actual
proteins, then do a in silica proteolysis with trypsin, input the results into
the database.
This approach is really database dominating. Not mention possible
post-translational modification and signal peptides and so on that could bring
a big trouble.


【 在 otis (孤鸿和小猪) 的大作中提到: 】
: Coming back from ASMS,I found many proteomics people identify proteins by
: LC-MS-MS.
: But what I know before is protein identification could be acchieved with
: peptides mapping without sequencing the protien.
:
: I also asked some people about that ,but different people come up with
: different answer.
:
: I am confused........
:
:

--
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