发信人: denovo (踩色神拍), 信区: Biology
标 题: Re: about primer
发信站: The unknown SPACE (Thu Jun 13 19:06:17 2002), 站内信件

From which company did you order your primer?
we order from Invitrogen, and they use different salt concentration
to calculate Tm. It's not about right or wrong, coz Tm changes with
oligonucleotide and salt concentrations, so u'd better calculate it by
yourself. Some softwares like Vector NTI can do it, I think Primer3
works too.

we use oligo conc. at 250 pM and salt conc. at 250 pM to calculate
the Tms.

【 在 DimSam (点心) 的大作中提到: 】
: I designed the primer by Primer3 and ordered it.
: But when the oligo arrived, the company gave the different
: Tm(62.5 C) from that by Primer3(60 C). Which one is correct?


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