发信人: Ras (很颓的猪。), 信区: Biology
标 题: Re: What method do you use for DNA transfection?
发信站: The unknown SPACE (Mon Jun 17 10:08:37 2002), 转信

yes, immortalized cell lines are generally harder to transfect
than primary cells.

however, 283 in general should be easy to transfect unless your
genes are really toxic to the cells (some of genes I have to use
are in such case).

Some guy upstair mentioned Superfect Reagent from Qiagen.
That one is quite effective, and better than LipofectAmine
in many cases, but more expensive too.


【 在 middlerain (不大不小，非雪非霜) 的大作中提到: 】
: Do you mean cell lines are easy to transfect?
: compaired to primary cells?
: I use 293 and 293T cells and mouse mycrophage cell for primary cell.
: Can you give some advices?
: 【 在 Ras (很颓的猪。) 的大作中提到: 】
: : It varies from cell line to cell lines a lot, and also depend
: : on the genes delivered into the cells. Some genes are just
: : expressed at very low level in cells.
: : primary cells are relatively harder to transfect.


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