发信人: webbie (笑面狐), 信区: Biology
标 题: Re: about RNA
发信站: The unknown SPACE (Thu Jun 20 22:30:35 2002) WWW-POST

It depends on how you want to use those RNAs.
I think if you try to do any reaction with them, it is not good to have a
trace
of ethanol. but if you want to extract mRNAs or do electrophoresis, it may not
matter.

BTW, have you tried to heat it at 65 degree for 10 minutes? it should help
dissolving RNAs.

The trizol protocol recommends to air dry RNAs for about 10 minutes, which
usually can results a little ethanol in the tube.

【 在 damulv (xiaomulv) 的大作中提到: 】
: Thanks.
: In fact,I washed RNA with 75% ethanol. I let the tube dry in air,but I added
: water to dissolve RNA as the ethanol is not dried up.
: Can I use these RNA? I don't want to extract RNA again.
:
:


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※ 修改:·webbie 於 Jun 20 22:30:35 修改本文·[FROM: 203.116.185.253]
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