发信人: Marble (小石头哥哥), 信区: Biology
标 题: Re: 一个7k长的cDNA
发信站: The unknown SPACE (Wed Jun 26 13:40:43 2002), 站内信件

this is a BIG problem. so what is the gene? and from what
kind of organism would u like to clone it? i have only a
couple of suggestions. first of all, check the RACE primers.
have you tried several different primers? 2nd, check the
known EST database and see whether there are severa image
clones available with which you can actually assemble the
clone. this gene might have very long 5'UTR. you may not
need the whole sequence for expression, though the UTR may
have regulatory sequences. 3rd, you may have to get or make
yourself a random-primed cDNA library. Clontech sells such
library for animals, if you know which tissue has enriched
this message. you may check RIKEN for mouse cDNA clones.
these guys work a lot. http://genome.gsc.riken.go.jp/
home.html
【 在 webbie (笑面狐) 的大作中提到: 】
: 现有3'约3k的序列，如何拿到全长？
: 5’RACE不出来。
: thanks


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