发信人: aroluna (出来乍到,多多关照), 信区: Biology
标 题: about hot-start PCR
发信站: The unknown SPACE (Tue Aug 20 13:55:28 2002), 站内信件

"Hot-start" PCR is a method that generally produces cleaner PCR products
. Template DNA and primers are mixed together and held at a temperature
above the threshold of non-specific binding of primer to template.
All the PCR reaction components are added for the extention reaction
except one critical reagent (usually the thermostable polymerase).
Just prior to the cycling, the missing component is added to allow
the reaction to take place at higher temperature. Due to lack of non-specific
hybridization of primers to template, the amplified DNA bands tend to be
cleaner; the primers don't have a chance to anneal non-specifically.


methods:
HotStarTaq DNA polymerase(qiagen所用)
Hot-start enzyme from Supplier AII
Antibody-mediated
Manual Wax
barrier

这是偶在前辈的提示下查到的,我明白一些了
3x

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※ 来源:．The unknown SPACE bbs.mit.edu．[FROM: 211.90.]