发信人: Tharu (待嫁的恐龙), 信区: Biology
标 题: Re: anyone working on RNA 2nd structure folding?
发信站: The unknown SPACE (Mon Aug 26 13:55:33 2002) WWW-POST

It really depends on the GC content of your RNA. My suggestion is to shuffle
and generate a random pool (say, 100) of your RNA based on the GC content.
Then run them through mfold (the server can handle batch jobs, you just need
to wait for a while), and then you will have a normal distribution of the
folding free energy. Compare the folding free energy of your native sequence
to the distribution will show you whether it's better or worse than random.
【 在 tlbb (天龙下凡) 的大作中提到: 】
: actually, let me just post the question here
: THe mfold program predicts a RNA piece (290 bp) to have one optimal
: structure with energy of -67.5 kcal/mole, my question is, how stable is
: this structure then? good, soso, poor?
:
:
: 【 在 tlbb (天龙下凡) 的大作中提到: 】
: : Need your help on a couple of simple questions, Thanks a lot. Please
: : send a note to my bbs email (ctrl-R), thanks
:
:

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