发信人: Marble (小石头哥哥), 信区: Biology
标 题: Re: RNA 的纯度问题
发信站: The unknown SPACE (Fri Aug 30 14:07:22 2002), 站内信件

i have never worked with plant, so i do not know whether this
could be due to sth that cuase higher obsorbance at 260nm.
the ratio for isolated RNA from animal tissues is usually 1.7-
2.0 for my experience, and it pretty much depends upon what
kind of kit/reagent i use. i try TRIZol (Invitrogen), which
is pretty good, but sometimes RNA from pancreas will degrade
with no mercy. i also use RNeasy Kit (Qiagen), which is said
to be better, particularly working for microarray purpose.
i would recommend you read the following thing from Ambion
http://www.ambion.com/techlib/tn/91/9113.html
Ambion is a good company that has virtually high tech and
experience dealing with various RNA. they have this RNAlater
Stabilization buffer, and RNA storage buffer, and i would
recommend you guys try that. for storage, in 70% ethanol
at -20 is good for short-term, while storage at -80 is for
long-term. i would use nuclease-free ddH2O (order it from
Ambion) rather than DEPC-treated ddH2O to dissolve RNA.
besides, make sure that you use RNase/DNase free eppendorf
tubes and tips (PCR tips with barrier should be fine).
【 在 jinghs (hh) 的大作中提到: 】
: 用TRIzol 从植物叶子里提取RNA,用分光光度计检测其纯度,OD260/OD280居然大于2.0.这
: 是怎么回事呀? 稀释度应该是合适的,因为读数在有效范围内.


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