发信人: Ras (很颓的猪。), 信区: Biology
标 题: Re: who has experience with PCR-based mutangenesis?
发信站: The unknown SPACE (Wed Sep 11 13:29:53 2002), 转信


It's called QuickChange Site-Directed Mutagenesis Kit.
Just seach stratagene website for it.

It's very easy to use. One thing is they usually give little
Pfu turbo compared to the other reagents from the kit. So you'd
better buy some extra Pfu turbo yourself if you are doing a lot of this.

【 在 golem (狗愣小岩) 的大作中提到: 】
: that one developed by Stratagene using DpnI to remove original plasmids.
: I plan to do some without buying the kit. If anybody has experience, please
: give me a list of critical steps and useful tips.
: thanks, and kow tow.


--
一年老一年，一日没一日，
一秋又一秋，一辈催一辈，
一聚一离别，一喜一伤悲。
一榻一身卧，一生一梦里。
寻一夥相识，他一会咱一会，都一般相知，
吹一回，唱一回。

※ 来源:．The unknown SPACE bbs.mit.edu．[FROM: 152.3.]