发信人: bongbongcat (cutie), 信区: Biology
标 题: Re: about conserved noncoding sequence
发信站: The unknown SPACE (Fri Oct 4 17:15:33 2002), 转信

kozac seq was found by a japanese lady whose name was kozac. she had a
single-author paper published in cell (in the 80s) describing a short
sequence around the start codon which gave rise to high level expression
in eukaryotes.

it is: ACCATGG. the "ATG" here is the start codon.

i always try to include this sequence when making plasmids for transfection.

【 在 middlerain (不大不小，非雪非霜) 的大作中提到: 】
: 【 在 Marble (小石头哥哥) 的大作中提到: 】
: : guess you have to learn more about this from a review paper.
: : think about all the conserved sequences in the promoter (e.g.,
: : TATA box, GC box, etc) and all the binding sites for the
: : transcription factors (Sp1, SREBP, C/EBP, etc), as well as
: : conserved sequences found in the 5' untranslated and 3'
: : untranslated regions (UTR) that is important (e.g.,
: : polyadenylation site, Kozac sequence, Shine-Dalgano in
: ~~~~~~~~~~~~~~ what's this?
: : prokaryote, PABP binding sites, sites for NMD, etc).


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※ 来源:．The unknown SPACE bbs.mit.edu．[FROM: 159.14.]