发信人: rmchen (Raymond), 信区: Biology
标 题: Keep your hands off 32P. Re: Is 同位素 32P very dangerous?
发信站: The unknown SPACE (Sat Oct 5 06:19:20 2002) WWW-POST
I have consulted with my supervisor and radiochemicals safety representative
in my lab. I was told to keep my hands off the eppendorf tube which contains
32p, even of a small quantity. It is a good practice to use forceps or tube
rack. DO NOT TOUCH THE TUBE EVEN FOR A BRIEF TIME.
Because I have to use 32P to label my protein and have to load my samples for
SDS-PAGE. So my finger was supposed to expose to 32p for quite a long
time(10min). I am scared by my doing when I retrospected my experiments.
Should I go to see a doctor about that?
【 在 Marble (小石头哥哥) 的大作中提到: 】
: i just did the experiment yesterday, i.e., in vitro labeling
: using gama-P32 ATP. glad that it worked for me. for the limbs,
: it is said that the harm from radioactivity is less severe than
: other part of the body, and that is why we can still handle
: the "hot" tubes. however, be sure to check the glove from time
: to time to eliminate any possible contamination, and keep
: changing it. monitor the place before and after you work, make
: sure to get rid of spills. every time after experiment, i also
: wash my hands and arms, though i wear glove and lab coat all
: the time. no abrasion, just rinse it with rapid torent of water.
:
: 【 在 rmchen (Raymond) 的大作中提到: 】
: : I have to use it for protein phosphorylation labelling. What should I pay
: : attention to? Thanks for any suggestion.
:
:
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※ 来源:.The unknown SPACE bbs.mit.edu.[FROM: 131.111.]
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