发信人: nondhyana (没事瞎嚷嚷), 信区: Biology
标 题: Re: 请教！
发信站: The unknown SPACE (Tue Oct 22 14:01:31 2002) WWW-POST

Yeast 2 hybrid can test protein--protein interaction( based on modular nature
of activator/ suppressors):

proteinA <--> proteinB+(AD) <-- Activation domain
+
(BD)<--DNA binding domain
-----------------------------------------------Reporter gene-----------DNA

Yeast 1 hybrid studies Protein--DNA interaction:


protein + (AD)
----------------------------Reporter gene---------------DNA


In 2 hybrid , fusion protein (pA+BD) binds to DNA(specific for BD binding),
but it does not have an activation domain(AD). So if pB can interact(bind)
with pA, then the whole complex can bind DNA and also contain an activation
domain which is fused to pB. Then Reporter gene expressed.

In 1 hybrid, Protein fused with an activation domain. If that protein can bind
to the seq. linked to the reporter gene, then the AD can activate the
transcription of the reporter.

I maight be wrong. But the above is what I remembered.


【 在 mystery (哇！好土的狼) 的大作中提到: 】
: what does yeast one hybrid mean?
:
: 【 在 wdgca (混在北京) 的大作中提到: 】
: : 偶用Yeast One-Hybrid的方法分离到几个蛋白质因子的cDNA。
: : 可能是植物里和抗胁迫（寒冷，干旱...)相关的转录因子，下一步想证明它在植物体
内的
: : 确切功能，该怎么做呢？没头绪的说，大虾救命！
:
:


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想吃豆腐，小心受不了！

※ 修改:·nondhyana 於 Oct 22 14:01:31 修改本文·[FROM: 132.235.]
※ 来源:．The unknown SPACE bbs.mit.edu．[FROM: 132.235.]