发信人: smileface (笑脸), 信区: Biology
标 题: Re: 检测基因表达变化时，RT-PCR和Northern blot首选哪个？
发信站: The unknown SPACE (Fri Dec 6 20:29:51 2002), 站内信件


clonist //hehe, take it easy.

generally, RT-pcr is the most sensitive assay to RNA, although sometimes
it cause pseudo positive.
However, RT-PCR can't detect isoforms, or alternative splicing products.
And because of its pseudo positive.
a northern is necessary.
However, S1 mapping of nuclease protection assay is roughly 20 fold more
sensitive than northern. Although these two methods also can't give
you the information of the full length or some isoforms' information.

all in all, these assays are complimentary to each other.
Depends on your aim, your project, what you have at hands.





【 在 clonist (chill out) 的大作中提到: 】
: wrong? should i believe the big guy in the field or you? hehe
: u even don't ask which field i'm talking about.
: 【 在 biology (生物) 的大作中提到: 】
: : that is absolutely wrong


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