发信人: royluo (roy), 信区: Biology
标 题: Re: freezing vials exploded!
发信站: The unknown SPACE (Mon Dec 16 20:59:01 2002), 站内信件
小MM被吓坏了。:-)
你LG怎么不教教你低温保存细胞的一些基本知识?
液氮渗入了小管之后,解冻的时候,液氮变为气象,
瞬间使管内压力增大700倍。所以发生爆炸。
不过你遇到的情况非常夸张,看起来液氮漏得很严重。
一般来说,只要你用非常专业的螺旋盖的低温保存管,
比如NUNC出的系列cryotubes,然后拧紧,不会出现任何
问题。
下一次再拿FREEZE DOWN的时候,注意带防护面具拉!
如果没有罩住整个脸的,只罩住眼睛的也行。主要是为了
保护眼睛。
【 在 shuanger (悲伤百合) 的大作中提到: 】
: today I opened a box which is immersed in liquid nitrogen,
: put my new vials in it,
: then several old vials in it exploded,
: the lids of them were in pieces,
: and the next second I felt like I was deaf coz it sounded like a real
: explosion.
: my husband said that is because some liquid nitrogen was trapped in the vials.
: u guys ever encountered this?
: so terrible, how can I avoid it next time, I remember I already closed them
: tightly.
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※ 来源:.The unknown SPACE bbs.mit.edu.[FROM: 129.49.]
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