发信人: smileface (笑脸), 信区: Biology
标 题: Re: 跨膜蛋白的orientation?
发信站: The unknown SPACE (Tue Dec 24 19:41:44 2002), 站内信件


(1)
fusiong protein with a enzyme digestion site. overexpress it and digest.
check media by western.
(2)
EGFP fused to N tel. microscopy
(3)
homology search of the N-ter from database.
(4)
I don't know. :)




【 在 odz (odd) 的大作中提到: 】
: 用什么方法能determine 一个蛋白的N端在膜外还是在胞内？
: 我的蛋白有300 KD, 是个跨膜蛋白。
: 我想到的方法是在体外培养的细胞中， 表达N 或 C 端fusion protein.
: then use FACS to cells whose fusion protein has its epitope outside.
: anyone has good suggestions? Thanks!


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