发信人: warrior (不死鸟), 信区: Biology
标 题: Re: 超声波裂解E.coli细胞求救！
发信站: The unknown SPACE (Wed Jan 15 22:26:35 2003), 站内信件

stir well, dip the tip fully in the cell suspend, use medium output,
a little foam is ok, don't be too nervous. I have done it hundreds of
times, protein is much tougher than you thing.
if there is no activity, it means there is not, not because of
the sonication. By the way, e coli is not that tough, don't sonicate too
long, and keep all your stuff in ice during the whole procedure. As of my
experience, heating is a bigger problem than foam.
【 在 wsdoll (doll) 的大作中提到: 】
: protocol说不要起泡，泡沫会对蛋白blah blah...但是我不论怎么试，都会起泡。怎么办
: 呢？
: 因为我最后要测提出来的蛋白的功能。以后如果做不出来，谁知道是不是裂解时就破坏了
: 活性亚？
: thanks a lot!


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