发信人: Marble (小石头哥哥), 信区: Biology
标 题: Re: 撕心裂肺地求救！！！
发信站: The unknown SPACE (Thu Jan 16 00:39:28 2003), 站内信件

dude, you scared me:P. to tell you the truth, nothing but
truth, i think so ... well, don't tell boss and buy another
vial then. next time, try not to do that. i suggest you use
a cell storage from Nalgene. add isopropanol into it, put in
the vial with cells (cells are in DMSO and FBS), and put it
@ -80C. it is said to decrease the temp by 1C/hr. after
put it in the -80C two days, you can now transfer it to
liquid N2. when you thaw the tube from N2, put it in 37C
water directly, don't stand outside too long.


【 在 special (xxxx) 的大作中提到: 】
: 正常血管内皮细胞增殖后，直接冻存在液氮里，几星期后，取出再次培养，细胞全部死亡
: ！！撕心裂肺的痛呀，花了几百刀买的细胞株难道就这样完了吗？师兄说我不应该直接把
: 细胞冻在液氮里，应先冻存于-80度冰箱里，几天后再转入液氮。现在不敢跟老板讲，大
: 家说我的细胞还有救吗？给出个主意吧。。。
: 顺便问一句，精液是不是直接冻存于液氮里？


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