发信人: reer (), 信区: Biology
标 题: Re: MCBJC, cell division, Jan 20 2003, by Assasin
发信站: The unknown SPACE (Mon Jan 27 02:04:48 2003) WWW-POST

【 在 oil (brother) 的大作中提到: 】
: let me try to say something, see if I understand it right.
:
: they claimed the 2 inhibitory functions on separase:
: 1. securin. no data on this part. assume it's well-known.
: and this seems have no in vivo function as shown by mutations
: (introduction) securin sequences are not conserved.
:
: 2. separase phosphorylation. then
: 2.1 what phosphorylate separase? ( did they show this clear?)
CDC2/cycB or Erk2 or other kinases, but they do exclude some kinase like
polo,auroraA and CaMKII by in vitro kinase assay.
: 2.2 effect of the phosphorylation ( this should be convincing)
: 2.3 this happens in vivo (maybe convincing?)
:
: I think the significance of this event is not fully revealed.
: (I mean genetics)
:
: 3. separase self cleavage? I can't find real data on this point.
they do show some data in fig2A, they mutated the catalytic site of
separase and found that separase can not be cleaved. "As the active site lies
far from where cleavage occurs, this result implies that the cleavage of
separase is autocatalyzed" Not quite convincible.
If you are interested, you can go read "anaphase specific auto-cleavage of
separase" done by same group.
:
: Please comment on these.
:
:


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