发信人: Marble (小石头哥哥), 信区: Biology
标 题: paper help/ call for MCBJC discussion
发信站: The unknown SPACE (Sun Feb 9 20:36:40 2003), 站内信件

folks, a lot of us may be busy. but if you are free and would
like to throw your quarters (usually coins, but the more, the
better), highly appreciate, since that will keep the MCBJC
going. and i think one good thing about MCBJC is that we can
definitely read some good articles and see how people in other
fields than yours work to address questions, and the ideas
might contribute to your project as well.
this Nat Genet paper was short, however, very informative. i
have not read it in detail, and what i can tell is that they
tried to address the cell-auntonomous function of Nf1 and
established the correlation of its function with the phenotypes
observed in the neurofibromatosis type 1 (NF1). Global knockout
of Nf1 results in mid-embryonic lethality and the cardiac
defects were previously attributed to be secondary to cardiac
neural-crest defects. in this work,two tissue-specific Cre
strains were used to address the questions; endothelial-specific
knockout mutations of Nf1 were generated and the phenotype of
cardiovascular abnormalities were recapitulated. meanwhile,
inactivation of Nf1 in the neural crest resulted in tumors
of neural-crest origin similar to that observed in NF1 human
patients. tissue-specific knockout mutations as well as
transgenics have been used extensively to address the cell-
autonomous function of interested gene and turns out to be
powerful genetic tool, particularly for mouse while nowadays
random genetic analysis is still not available.

btw, see if you can do me a favor and forward two papers from
Neuron. 1998 21:1243, and 1999 22:103 from Scott MP, to my
mbox [email protected] (one by one, pls:).

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※ 来源:．The unknown SPACE bbs.mit.edu．[FROM: 128.118.]