发信人: smileface (笑脸), 信区: Biology
标 题: Re: transcription activator -> regulated genes
发信站: The unknown SPACE (Thu Mar 6 22:13:45 2003), 转信



there are several ways you can think about, although techniquely,
they are quite difficult.
(1) If you know what is the transcription activator, there should
have a databank contain promoter sequences. (Sorry, I can't remember
the webste)

(2) You can use CHiP analysis to clone out DNA sequences that bind
to your protein. First crosslink, then sonicate, then immunoprecipitation,
then clone and sequence, then search database.

(3) As u need those genes regulated in the early 4hrs. Tranditional
overexpression many be harsh. You estable stable cell line with Tet-on/off
system. Or directly transfect cells with purified proteins with some
commercial reagents.
(a) microarray system
(b) RDA, substraction hyb, SAGE, DD-PCR....... quite a lot here.
to compared to your controls.








【 在 laomeng (LaoMeng) 的大作中提到: 】
: Need some help:
: Given a transcription activator or an upstream signaling molecule (i.e. Elk-1
: or p38), find a list of all genes that this protein regulated in the first
: 4 hours ...
: Sequence point of view, I can search all gene sequence with promotor binding
: sequence if available, but 1. hard to locate promoter region 2. less sequence
: info of promoter region.
: Tried to use traditional literature search, but it seems taking me already
: too long.
: Any suggestion? //bow


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