发信人: sunnyday (飞鸟), 信区: Biology
标 题: Re: 问问关于lysis buffer
发信站: The unknown SPACE (Thu Mar 20 23:07:10 2003) WWW-POST

This is a convenient recipe: 0.5% Triton X-100, 20~50mM Hepes-KOH, pH 7.8,
50mM KCl, 2mM MgCl2, 5mM EGTA,1mM EDTA, 100mM Sucrose, Plus cocktail of
protease inhibitors.
Triton X-100 is for lysis of cell membrane, Sucrose is for the stablity of
most proteins, protease inhibitors is again proteolysis.
However, this may differe depend on your purpose. You should check related
reference to get a recipe that works for your experiment
By the way, in some recipe, I notice they don't use Triton, don't understand
why, anyone knows?

: 【 在 MangaII (忙啊) 的大作中提到: 】
: : 我要裂解小鼠Brain，并要把细胞核也裂解掉，但是蛋白不能变性，因为要拿来做
: : Pulldown。
: : Buffer里面有什么要特别加地吗？或者有啥Lysis Buffer配方。
: : 情做过地大侠指点，谢谢
:
:


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