发信人: Smeagol (Gollum), 信区: Biology
标 题: Re: PCR产物纯化方法
发信站: The unknown SPACE (Wed Apr 2 19:33:21 2003), 站内信件

it's actually centrifugal filter instead of column...
here
http://www.millipore.com/catalogue.nsf/docs/C3034
for 500bp dsDNA, seems u can use the largest, YM-100.

for better purification, u can also use microcon PCR
I think it's now called Montage PCR Filter, here
http://www.millipore.com/catalogue.nsf/docs/C7480

Good luck:)

【 在 julieni (julie) 的大作中提到: 】
: 谢谢!
: 不好意思我是新手.还是有些不明白的地方.
: 请问这种column是不是,一个tube里套着一个带滤膜的小tube的东东,
: 英文名字是"column centrifuge"?
: 这种column有很多size吗,我的product有500bp左右,哪个公司可以order?
: 【 在 Smeagol (Gollum) 的大作中提到: 】
: : microcon PCR or microcon from millipore
: : very convenient :)
: : just load your solution in the column (actually a membrane)
: : centrifuge, PCR product hold on the membrane
: : then add water (the volume u want)
: : invert your colume
: : centrifuge again
: : yield is quite high
: : be sure to choose the right one according to your product size tho


--

Yess we waterss, my preciousssss


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