发信人: Marble (小石头哥哥), 信区: Biology
标 题: Re: 单链核苷酸脱盐，用啥柱子好？
发信站: The unknown SPACE (Fri May 9 13:13:40 2003), 站内信件

you are right. the difference is exclusion size. protein or
molecule that has bigger size than 25kD globin protein cannot
enter G25, and thus pass through the column without delay. the
oligos are pretty small and can be held in both G25 and G50
columns. so why do they recommend G25 instead of G50?

【 在 sunnyday (飞鸟) 的大作中提到: 】
: 【 在 Marble (小石头哥哥) 的大作中提到: 】
: : both are fine for desalting. check the cut-off range though,
: : for G25, it is smaller and definitely safer for your small
: : nucleotides (give it enough time to hold the oligos).
: ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
: I am afraid this is a misconception. G-25 and G-50 both allow small
: molecule get in the gel particle with no problem, the difference is
: the way bigger molecule pass through them.
: : wash the column with enough HPLC grade water, load the sample
: : in small amount of buffer (or dilute in larger volume of water),
: : and then collect the flow through. however, i am thinking about
: : that you still need to concentrate the oligos (would spin dry take
: : too long?) btw, why didn't you order desalted oligos?
: : btw, paper help bah:
: : Neuron 2002 Feb 28;33(5):715-29
: : to [email protected]
: 盐的
: 是不
: 一种30个base。需要用G-25的还是G-50的？


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