发信人: Marble (小石头哥哥), 信区: Biology
标 题: Re: 厚着脸皮问一句：1 OD到底对应多大浓度的单链DNA溶液？
发信站: The unknown SPACE (Wed May 14 17:39:45 2003), 站内信件

make sure that you are aware of the buffer effect:
It is estimated that DNA has 15% lower absorbance in TE
buffer, 23% lower in TE+saline compared to water.
1 O.D.260 are 38, 45 and 50 ug/ml for DNA in ddH2O,
TE and TES, respectively. I assume the same for RNA,
and 1 O.D.260 are 40 ug/ml for RNA in TE (from Ambion).

btw, A260/A280 is different for pure RNA
DEPC'd H2O (pH5-6) 1.60
Nuclease free H2O (pH6-7) 1.85
TE (pH8.0) 2.14

【 在 gdma (胖妞儿) 的大作中提到: 】
: 俺知道这个问题够弱智。各位大侠看在俺半路出家的份上，就吭一声吧。我用google查，
: 有的网页上说40ng/ul，有的说33ng/ul，俺也不知道哪个对。
: 另外哪位能推荐一本好的实验技术手册，就是能让俺查出来这种弱智问题的答案的那
: 种。不胜感谢。


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※ 来源:．The unknown SPACE bbs.mit.edu．[FROM: 128.118.]