发信人: gooddream (damn, damn, it is not right), 信区: Biology
标 题: 3 ways Re: 请问怎样浓缩浓度过
发信站: The unknown SPACE (Thu Jun 19 14:53:16 2003) WWW-POST

A) Ethanol precipitation
1)Add to the DNA solution 1/10 volume of 3M NaOAc and 3 volume of EtOH
2)Leave in the -70 degree freezer for 20 minutes
3)Spin in microcentrifuger for 10 minutes to pellete DNA
4)Wash with 70%ethanol and spin for 10 minutes
5)Air-dry the DNA and resuspend in water,Tris buffer or TE buffer

B) Isopropanol precipition
1)Add to the DNA solution 1/2 vol.of 7.5 M NH4OAc and 2 vol. isopropanol
2)Incubate at room temperature for 10 minutes
3)Spin for 10 minutes.Wash with 80%ethonal
4)Air-dry DNA and resuspend in water or buffer of choice

C) Precipitation with glycogen
Use 1 uL of 20 mg/ml stock to DNA solution up to a vol. of 1 mL







【 在 teddybear (~_~) 的大作中提到: 】
: 提取的细菌genomic DNA溶解在100mM TE buffer中，定量时发现浓度太低，请问怎样浓
缩
: 呢？再用isoproponol沉淀DNA能析出吗？因为细菌样品量有限，所以很着急，恳请有经
验
: 的xdjm 帮忙.thanks a lot!
:
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