发信人: clonist (dream on), 信区: Biology
标 题: Re: mammalian transgene expression
发信站: The unknown SPACE (Mon Jul 14 13:45:10 2003), 站内信件

for a long time people already know that the presence of an intron will
signicantly increase the expression level of genes. the classic example is
beta-globin. w/o intro its cDNA barely expresses in cells. with an intron
even an irrelevant intron (insulin intron for example) it expresses at very
high level. you may wanna search with key word "intron, expression (beta-globin)
but i don't know much what's the situation in transgenic mice though.
【 在 Cathleen (蛹-@-化蝶) 的大作中提到: 】
: You didn't get what I said,the concept is that the intron is required to put
: together with the transgene otherwise the transgene may not be expressed in
: the transgenic mice, more about this concept is that better no intron with the
: transgene if it's for tissue cultured cell expression.
: 【 在 merben (merben) 的大作中提到: 】
: : so when you get your transgenic mice, you can detect the transgene
: transcript
: : level through RT-PCR, because the intron should be cut out.
: : is that making sense?


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※ 来源:．The unknown SPACE bbs.mit.edu．[FROM: 152.16.]