发信人: Cathleen (蛹-@-化蝶), 信区: Biology
标 题: Re: mammalian transgene expression
发信站: The unknown SPACE (Mon Jul 14 18:47:18 2003) WWW-POST

yeah apparently beta-globin transgene expression is intron-dependent,but other
genes maybe not, actually my lab and another lab in the same floor each got a
transgenic mice line with desired expression level w/o adding any introns.

I'm using the same vector pTRE as they did,maybe not that risky, should I add
an intron to my construct??

【 在 clonist (dream on) 的大作中提到: 】
: for a long time people already know that the presence of an intron will
: signicantly increase the expression level of genes. the classic example is
: beta-globin. w/o intro its cDNA barely expresses in cells. with an intron
: even an irrelevant intron (insulin intron for example) it expresses at very
: high level. you may wanna search with key word "intron, expression
(beta-globin)
: but i don't know much what's the situation in transgenic mice though.
: 【 在 Cathleen (蛹-@-化蝶) 的大作中提到: 】
: : You didn't get what I said,the concept is that the intron is required to
put
: : together with the transgene otherwise the transgene may not be expressed
in
: : the transgenic mice, more about this concept is that better no intron with
the
: : transgene if it's for tissue cultured cell expression.
: : 【 在 merben (merben) 的大作中提到: 】
: : : so when you get your transgenic mice, you can detect the transgene
: : transcript
: : : level through RT-PCR, because the intron should be cut out.
: : : is that making sense?
:
:

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