发信人: clonist (dream on), 信区: Biology
标 题: Re: mammalian transgene expression
发信站: The unknown SPACE (Mon Jul 14 20:25:50 2003), 站内信件

gee, poly(A) will contain an intron? you mean 3' UTR ba. there
are some commerical available vectors containing an intron. as
far as i know they usually put the intron at 5'UTR. coz you have
to pay attention to NMD. when you put an intron at 3'UTR it
becomes a classic example of NMD if your intron locates more than
50-55nt downstream of the stop codon.

【 在 Cathleen (蛹-@-化蝶) 的大作中提到: 】
: I'm hoping that the polyA signal part of the pTRE vector contains an intron.
: I called ClonTech,from which this vector is oringinally from,but they don't
: know.:(
: where and how to get an intron????
: 【 在 clonist (dream on) 的大作中提到: 】
: : well, it's case by case situation here. not dependent on your vector but the
: nature
: : of your gene. genearlly speaking adding an intron won't hurt. also you can
: do
: : a transient transfection to test if desired.
: other
: got a
: add
: is
: intron
: very


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