发信人: triangle (try), 信区: Biology
标 题: Re: what is p24 infection?
发信站: The unknown SPACE (Thu Jul 24 14:25:10 2003), 站内信件

of course those plasmids will produce enough hiv particles. like the classic
pNL4-3 WT. but for a lot of reasons, either bio-safty or their application,
people tend to use plasmids with partial defective HIV genome, such as Rev-
Tat-, Env- etc. then supply the corresponding plasmid encoding Rev, Tat, Env or
sometimes VSV G coat protein to fully rescure HIV replication and make infectious
HIV viral particles. although those virions are fully infectious they by themselve
will not produce infectious next generation particles coz they contain defective
genome. thus you'll be sure only one infection happens and it's much safer than
working with wild type.
【 在 else (where) 的大作中提到: 】
: 【 在 triangle (try) 的大作中提到: 】
: : normally people transfect cells with HIV encoding plasmids, couple of days
: later,
: : measure the p24 level in the supernatant medium. its read-out will be xxx
: ng/ml
: : then u decide how much supernatnat u use to infect your cells.
: Thank you so much! I have got the sense. Nevertheless, concerning what you
: said, I wonder if there is such plasmid producing viron. I think people are
: using recombinant virus instead of plasmid.


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