发信人: happyzhb (~~享受中~~), 信区: Biology
标 题: Re: 大虾救命
发信站: The unknown SPACE (Mon Jul 28 22:11:43 2003) WWW-POST

据我所知加sodium acetate just help precipitate DNA, any salt should work. So
your DNA should be ok.

10% Sodium Deoxycholate(M = 414.6) has lower amount of salt than 3M sodum
acetate(M = 82.03), thought precipitation should not as good as adding sodium
acetate, wierd?

But your DNA should be ok, just re-solve it and purify again, should be fine.

Only I don't know if deoxycholate will cause any unexpected reaction. hehe!

Good luck!


【 在 dh (dh) 的大作中提到: 】
: 今天急着回家，结果犯了个很愚蠢的错误，不知还能不能弥补。一个很重要的sample(
是
: 偶过去两个星期的心血)用phenol-chloroform extract DNA.After
: Chloroform:isophrophonal 24:1 save the top phase to a new tube， I add 20 ul
: 10% Sodium Deoxycolate instead of 3 M sodium acetate. And I saw some
: precipitation when add 100% ethanol (more pellet than it should be)Can i
still
: use the pellet to do the PCR or run on the Gel? 鞠躬了
:
:

--
give up!
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