发信人: leohawk (leohawk), 信区: Biology
标 题: Re: 有用anti-GFP Ab做肿瘤组织染色的吗？
发信站: Unknown Space - 未名空间 (Thu Sep 11 15:08:43 2003), 转信

pre-absorption might help you, just use tumore sample without GFP to
pre-absorb your antibody before you use it on the real tissues.

you can also increase the stringency of your experimental condition, like
increase BSA concentration or Tween concentration, wash more rigorously.

I once made affinity-purified anti-RAD51 myself, however, I can't use it for
anything since it binds to a lot of other tissues. Wish you good luck


【 在 xiaomimiduck (xiaomimi) 的大作中提到: 】
: 我用表达GFP的病毒注射肿瘤(in mice)，取出肿瘤组织做切片，然后用FITC conjugated
: anti-GFP antibody(goat) 染色，结果组织中用很多auto flurosence，搞不清楚到底那
: 些是特异的那些是非特异的，各位高人给帮帮忙吧，谢了。


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