发信人: glider (大苹果), 信区: Biology
标 题: Re: Sequence
发信站: Unknown Space - 未名空间 (Mon Oct 13 17:20:53 2003), 站内信件
Oh, I omit an E
it is I.M.A.G.E. -you can check the clone out in ncbi, to see if there is information
on this cDNA/entry, if there is something like an image number, or atcc number
then you can find the clone from atcc website, order it, about $50~ bacteria ...
【 在 windysea (雨瞳~像一个小孩只懂在你怀里坏) 的大作中提到: 】
: as I said, they are same mismatches on both pcr products and the original one.
: I was using pfuUltra HF in the kit since I was doing site directed mutagenesis
: instead of cloning. pcr should not be the problem, since it only introduced
: the
: designed mutation for mutagenesis. Other mismatches are also present in the
: original one.
: Can you give me the information for IMAG? anyway, I doubt if it has it since
: this one is big and relatively novel.
: 【 在 glider (大苹果) 的大作中提到: 】
: : it is possible the gene in the bank has mistakes
: : but what pcr enzyme did you use, since taq tends to give errors
: : besides, you may order the clone from IMAG directly, instead of cloning
: : by yourself, if you are sure the clone is the one that you are going to use
: : I guess it is cheaper to buy the clone, than do the whole cloning/sequencing
: by your own
: they
: all
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※ 来源:.Unknown Space - 未名空间 mitbbs.com.[FROM: 128.59.]
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