发信人: tianyu (tttt), 信区: Biology
标 题: Re: 变态老师要求用EB染胶
发信站: Unknown Space - 未名空间 (Tue Oct 14 02:14:38 2003), 站内信件

To have a nice gel (and more precise sizing), you need to run the
gel without EB. To teach a method, I think your TA did a right thing.

If you only need a quick a dirty way to check the nucleic acid, it is
OK to have EB in the gel.

BTW, EB stains different types of DNA with different efficiency, so
longer staining/destaining might be necessary.


【 在 bibo (清粥小菜) 的大作中提到: 】
: 我们TA得老师，不让把EB直接加到胶里面去，要求染色，脱色。
: 害得我跑2块胶一共从下午4点到晚上9点。
: 他们学生这个都不做，都不知道学些什么。
: 下回一定不能听她话。
: 气死人了。


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※ 来源:．Unknown Space - 未名空间 mitbbs.com．[FROM: 68.52.]