发信人: doggiebear (doggiebear), 信区: Biology
标 题: Re: 1st paper --random journal club
发信站: Unknown Space - 未名空间 (Sun Oct 19 23:47:56 2003) WWW-POST

I just briefly go through the paper, not look into detail of many gel
pictures yet. It is a very interesting paper. Basically the authors found that
NO can inhibit the exocytosis of Weibel-Palade bodies (by endothelial cells to
mediate thrombosis and inflammation). the mechanism is that NO will
nitrosylate the cysteine residues on NSF, which blocks its SNARE complex
diassembly function.
I am not familiar with this process. Just have several questions.


1) how does NSF diassemble the SNARE complex? seems this is an ATP hydrolysis
depedent process. Is it kind of motor that mechanically seperate the four
helical boundle of the complex?

2) When NO nitrosylate the NSF, how does it make sure it only nitrosylate the
cysteine residues on NSF, not on other proteins? Are there any recognition
process involved? Other protein involved? in virtro experiment done in this
paper seems did not have other proteins. But how about the in vivo situation?


3) NSF affect vesicle traffic everywhere in the cell, not just the
Weibel-Palade body. How does the cell make sure NO only affect the
Weibel-Palade exocytosis process? Or does it?


--
I will vote a democrat even he is a dog.
Liberalism is something to be proud of!
※ 修改:·doggiebear 於 Oct 19 23:47:56 修改本文·[FROM: 208.180.]
※ 来源:．Unknown Space - 未名空间 mitbbs.com．[FROM: 208.180.]