发信人: moso (野鬼), 信区: Biology
标 题: Re: totalRNA isolation.
发信站: Unknown Space - 未名空间 (Fri Dec 26 23:08:47 2003), 转信

I am not so sure if RNase keep active in such a solution containing 50% or so
isopropanol. However, For purification purpose, it is not suggested to have a
nuclear acid precipitated with isopropanol method either under low temperature
or for long incubation. Salts often have lower solubility in isopropanol
solution than in ethanol solution.

【 在 zhenzhengniu (H2S) 的大作中提到: 】
: In theory, infinitely.
: But in reality, due to the existence of RNase, 5-15 minutes with lower
: temperature.
: 【 在 merben (merben) 的大作中提到: 】
: : During the procedure of isolating totalRNA from cells, after adding
: : isopropanol to the supernatant, how long the RNA can stay in isopropanol
: : solution?
: : thanks.


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