发信人: nooodle (少爷), 信区: Biology
标 题: Re: HELP: how to select RNAi target sequ
发信站: Unknown Space - 未名空间 (Sun Jan 4 00:25:24 2004) WWW-POST

There are two very important reports in recent Cell discussing how to design
potent siRNA.One is from Zamore lab, the other from Amgen company. Basically,
the rule is to put a weak base pair, for example A-T or even mismatch with
target, at the 5'-end of the antisense strand of siRNA. Such a design
faciliates RISC selecting the antisense strand as guide strand, instead of
sense strand. Most of previous failed siRNA sequences follow the opposite of
rule. Given this discovery, other considerations, such as CG content,
secondary structure of RNA, may not be essential for designing a good siRNA.

【 在 smallstone (小丸子) 的大作中提到: 】
: Does it matter which region you target, i.e. c-terminal or N-terminal or
UTR?
: And does GC content affect the result?
:
:


--
※ 来源:．Unknown Space - 未名空间 mitbbs.com．[FROM: 172.132.]