发信人: cDNA (运动狂魔之岁月蹉跎), 信区: Biology
标 题: Re: HELP: how to select RNAi target sequence
发信站: Unknown Space - 未名空间 (Sun Jan 4 02:38:21 2004), 站内信件

from the work of my hand:
genarally speaking, select blindly you will get 1 out of 2 working, 1 out of
5 will working really good ( >80% knock down)

But i also know a guy select 19 sites, and none of them work.. hehe,
poor guy...


【 在 middlemouse (mikey) 的大作中提到: 】
: It is very hard to say. I saw some papers said everywhere is OK. But some
: papers said, only some region worked.
: 在 smallstone (小丸子) 的大作中提到: 】
: : Does it matter which region you target, i.e. c-terminal or N-terminal or
: UTR?
: : And does GC content affect the result?


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