发信人: cDNA (运动狂魔之岁月蹉跎), 信区: Biology
标 题: Re: HELP: how to select RNAi target sequ
发信站: Unknown Space - 未名空间 (Sun Jan 4 02:42:36 2004), 站内信件

【 在 nooodle (少爷) 的大作中提到: 】
: There are two very important reports in recent Cell discussing how to design
: potent siRNA.One is from Zamore lab, the other from Amgen company. Basically,
: the rule is to put a weak base pair, for example A-T or even mismatch with
: target, at the 5'-end of the antisense strand of siRNA. Such a design
: faciliates RISC selecting the antisense strand as guide strand, instead of
: sense strand.

the key part is difference, if the 3'end even weeker, that will not work.
and mismatch with target have nothing to do with this issue.

:Most of previous failed siRNA sequences follow the opposite of
: rule. Given this discovery, other considerations, such as CG content,
: secondary structure of RNA, may not be essential for designing a good siRNA.

not really... hehe... :)


: 【 在 smallstone (小丸子) 的大作中提到: 】
: : Does it matter which region you target, i.e. c-terminal or N-terminal or
: UTR?
: : And does GC content affect the result?


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