发信人: adeno (天夺其魄), 信区: Biology
标 题: Re: CaP transfection
发信站: Unknown Space - 未名空间 (Sat Feb 7 17:41:44 2004) WWW-POST

Try that alternative protocol in current protocols in molecular biology which
use BES instead of HEPES.I got much higher efficiency than with HEPES, and I
didn't find any obvious toxicity.

Toxicity may also be cell type specific. Regular HEK293 and Hela cell which I
used seem all right with CaP

【 在 raison (raison) 的大作中提到: 】
: anyone done CaP transfection successfullly?
:
:
: i tried according to this protocol
:
: http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/Ca_P_TX_R.shtml
:
: the transfection efficiency is much higher than expected, but the toxicity
is
: unpleasantly high as well. around 1/3 of cell death.
:
: do you guys have any suggestions? thanks
:
:

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