发信人: LecEgf (no+sugar), 信区: Biology
标 题: Re: 请教关于CRYSALIZATION
发信站: Unknown Space - 未名空间 (Wed Feb 18 16:25:56 2004) WWW-POST

a good start. The next stage would be trial-and-error based experiments. The
commonly used ways (in order of my perference):

change your construct, delete some residues from N or C terimus (strongly
suggested, some times a few residues make a huge difference)
change protein concentration
change temperature and try to find another hit
seeding..................

【 在 magie (*^*) 的大作中提到: 】
: 我最近开始CRYSALIZE一个蛋白,
: 试了HAMPTON SCREEN I&II 之后,有一个HIT, 但是形态是许多薄片叠在一起,没一片都
太
: 薄,也很难分开. 基于那个HIT,试了其他溶液浓度和PH,又试了75个ADDATIVE
: SCREEN之后,拿到的HIT还是那种形态的.
: 请问谁知道有什么办法避免这种情况,从而得到单个的晶体哪?
: 多谢
:
:


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