发信人: moso (野鬼), 信区: Biology
标 题: Re: How long does it take for expression
发信站: Unknown Space - 未名空间 (Tue Mar 2 17:33:22 2004), 转信

The time for assay your transient expression depends on your host cell, transcrioption
effeciency,posttranslation mechanism etc. In one words, there are many may have
effect on your expression assay. So people used to do a progressive assay to
determine the best time point for your assay.
According to different transfection protocol, there is difference of incubation
time before conducting the assay.
The mechanism of transfection between Lipo and Calcium is diferent. For the
different cases, the effeciency between the two methods varys.



【 在 glider (大苹果) 的大作中提到: 】
: How long does it take for transfected gene to be expressed in mammalian cells
: after transient transfection?
: In my lab, some people took 36-48 hrs, then do the assays, some people using
: 24 hours... Furthermore, does different method need different time of
: incubation?
: what is the difference between lipofectamine 2000 and calcium phosphate
: transfection in this aspect?
: It would be great if you can share your experience!


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